Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye
نویسندگان
چکیده
The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L, temperature 24 °C) around 76% of dye decolorization was achieved. Reusability study showed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage.
منابع مشابه
Evaluation of Dye Compounds’ Decolorization Capacity of Selected H. haematococca and T. harzianum Strains by Principal Component Analysis (PCA)
The selected strains of microscopic fungi, Haematonectria haematococca (BwIII43, K37) and Trichoderma harzianum (BsIII33), decolorized the following monoathraquinone dyes with different efficiency: 0.03 % Alizarin Blue Black B, 0.01 % Carminic Acid, 0.01 % Poly R-478, and 0.2 % post-industrial lignin. The most effective was the removal of 0.03 % Alizarin Blue Black B (50-60 %) and 0.01 % Carmin...
متن کاملApplication of immobilized horseradish peroxidase for removal and detoxification of azo dye from aqueous solution
The development and application of an immobilized horseradish peroxidase (HRP) on calcium alginate gel beads are reported for the successful and effective decolorization textile industrial effluent. The optimal condition for the immobilization of HRP on calcium alginate gel beads which leads to a specific activity of 15 U/g calcium alginate was identified. Upon immobilization, considering enzym...
متن کاملAnthraquinone dyes decolorization capacity of anamorphic Bjerkandera adusta CCBAS 930 strain and its HRP-like negative mutants
Cultures of the anamorphic fungus Bjerkandera adusta CCBAS 930 decolorizing, in stationary cultures, 0.01 % solutions of carminic acid and Poly R-478, were characterised by a strong increase in the activity of the horseradish peroxidase (HRP-like) and manganese-dependent peroxidase (MnP) at a low activity of lignin peroxidase. Genotypically modified mutants of B. adusta CCBAS 930: 930-5 and 930...
متن کاملImmobilization of Peroxidase onto Magnetite Modified Polyaniline
The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the pa...
متن کاملSandal reactive dyes decolorization and cytotoxicity reduction using manganese peroxidase immobilized onto polyvinyl alcohol-alginate beads
BACKGROUND Fungal manganese peroxidases (MnPs) have great potential as bio-remediating agents and can be used continuously in the immobilized form like many other enzymes. RESULTS In the present study, purified manganese peroxidase (MnP) enzyme isolated from Ganoderma lucidum IBL-05 was immobilized onto polyvinyl alcohol-alginate beads and investigated its potential for the decolorization and...
متن کامل